| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Palm Bay |
A | WAMSLEY | McCary | Walker | Henderson | DNS | 5:00.88 2025 Heritage Invitational 2 |
| 4 | Holy Trinity |
A | Mooney | Maaser | Young | McCarley | DNS | |
| 5 | Melbourne Central Catholic |
A | White | Davis | Kling | Conlon | DNS | |
| 6 | Rockledge |
A | Waller | Vereen | Jhagroo | Dupuch | DNS | |
| 7 | Heritage |
A | Clarks | Ferguson | Jean-Louis | Melendez | DNS | Cape Coast Conference Championship 2025 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Satellite |
B | Carver | Cash-Ianello | Hester | Thomforde | 4:40.00 | |
| 2 | Pineapple Cove |
A | Ahmad | Rosado | Revis | Goddard | 4:25.59 | FHSAA 1A District 8 |
| 3 | Satellite |
A | Harris | Merrick | Coryell | Williams | 4:12.00 | |
| 5 | Viera |
A | Bright | Church | Soto | Wagner | 4:10.57 | FHSAA 4A Region 2 |
| 7 | Melbourne |
A | Toenjas | Tobia | Ulrich | Garnto | 4:40.00 |