| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | West Shore |
A | Villanueva | Venkat | Matias | Johnson | DNS | Tiger Invitational 2026 |
| 3 | Pineapple Cove |
A | Sanchez | Borland | Granados-Garcia | Garcia | 47.20 | Rockledge Raider Classic |
| 4 | Cocoa Beach |
A | Mohr-Bollinger | Varis | Henderson | Maas | DNS | FHSAA 2A District 7 |
| 5 | Edgewood |
A | Jaksetic | Sriramineni | Nicholls | Caple | 46.90 | 2025 Merritt Island Relays |
| 6 | Astronaut |
A | Potter | Sherman | Rainey | Davis | 47.58 | Viera High School Invitational |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Palm Bay |
A | Andrews | Sedaros | Nedd | STAFFORD | 45.00 | 2026 Heritage Invitational 1 |
| 2 | Sebastian River |
A | Marshall | Gibbs | Gibson | St. John | 44.15 | FHSAA 3A Region 4 |
| 3 | Viera |
A | Mattos | Peeples | Jean | Pulliam | 43.17 | Viera High School Invitational |
| 4 | Melbourne Central Catholic |
A | Crockett | Clarke | Bronenbeger | Flores | 41.81 | 41.81 2025 Melbourne Central Catholic Invitational |
| 5 | Heritage |
A | Owens | Greer | Copeland | Simpkins | DNS | FHSAA 4A District 7 |
| 6 | Melbourne |
A | Patrick | Hall | Suro | Stanley | 44.00 | |
| 7 | Holy Trinity |
A | Pedicini | Spencer-Rodriguez | Jackson | Bagwell | 45.00 | |
| 8 | Eau Gallie |
A | Cook | Bracetty | Colpetzer | Official | 45.12 | 2026 Heritage Invitational 1 |