| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Melbourne |
A | Dieuveille | Petersen | Hedgespeth | Alvarez | 3:52.00 | |
| 3 | Melbourne Central Catholic |
B | Crockett | Bronenbeger | Semwayo | Bronenbeger | NT | |
| 4 | Pineapple Cove |
A | Nina | Lucas | Granados-Garcia | Guerra | 3:41.70 | FHSAA 1A District 8 |
| 5 | Edgewood |
A | Jaksetic | Sriramineni | Nicholls | Caple | 3:42.28 | 3:42.28 Rockledge Raider Classic |
| 6 | Satellite |
B | Betancourt | Payne | Payne | Colangelo | 3:50.00 | |
| 7 | Astronaut |
A | Ebaugh | Robinson | Quijada | Crick | 3:51.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Melbourne Central Catholic |
A | Smith | Brown | Williams | Nail | DNS | 3:44.71 2025 Melbourne Central Catholic Invitational |
| 4 | Rockledge |
B | Manning | Johnson | Raymundo-Wulf | Crouch | DNS | |
| 5 | Merritt Island Christian |
A | Bryant | Edalgo | Campbell | Schermbeck | DNS | |
| 6 | Palm Bay |
A | Loach | STAFFORD | Lockhart | Stalter | DNS | FHSAA 2A District 7 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Sebastian River |
A | Flemming | Smith | Mewborn | Gibbs | 3:38.77 | 3:38.77 2025 Merritt Island Relays |
| 2 | Rockledge |
A | Gonzalez | Butler | Flowers | Gordon | 3:35.00 | |
| 3 | Cocoa Beach |
A | Jackson | Cerveny | Reyes | Venzara | 3:27.27 | 3:27.27 Cape Coast Conference Championship 2025 |
| 4 | Viera |
A | Tud | Paul | Thompson | Pulliam | 3:25.72 | FHSAA 4A Region 2 |
| 5 | Heritage |
A | Greer | Seraphin | Berrios | Cruz de Leon | DNS | FHSAA 4A Region 2 |
| 7 | Holy Trinity |
A | Abdurahim | Bagwell | Barochia | Rector | 3:35.00 | |
| 8 | Satellite |
A | Kasza | Gelfond | Nolte | Saxe | 3:40.00 |