| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Clopton Middle School | A | Waller | Hall | Wilson | Salmons | 2:06.14 | Verified |
| 3 | Winfield MS | B | Ash | Davis | Spraggon | Cruse | 1:55.94 | Verified |
| 4 | Black Hawk Middle School | B | Gibson | Covert | Kurtz | Barks | 1:46.42 | Verified |
| 5 | Black Hawk Middle School | A | Forrest | Sanders | Jackson | Reel | 1:46.42 | Verified |
| 6 | Winfield MS | A | Beck | Callaway | Phelps | Shinn | 1:55.94 | Verified |
| 7 | St. Louis Blue Knights Middle Sc | A | Ward | Jacobs | Hughes | Miller | 2:37.39 | Verified |
