| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Louis Blue Knights Middle Sc | A | Muratori | Mallonee | Krestel | Bryant | 4:43.73 | Verified |
| 4 | Black Hawk Middle School | A | Sanders | Jackson | Peth | Bowles | 4:31.75 | Verified |
| 5 | Wright City MS | A | Baugher | Baugher | Lehmen | Love | 4:33.42 | Verified |
| 6 | Winfield MS | A | Callaway | Marsten | Shinn | Shoemake | 5:12.73 | Verified |
