| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Winfield MS | A | Creamer | Liberson | Malloy | Rau | 5:59.00 | Verified |
| 3 | Silex Middle School | A | Harris | Schwendemann | Grateke | Mehler | 5:22.90 | Verified |
| 4 | St. Louis Blue Knights Middle Sc | A | Criswell | Plymale | Clark | Brown | 4:48.07 | Verified |
| 5 | Black Hawk Middle School | A | Sebastian | Duncan | Faubion | Donovan | 5:19.14 | Verified |
| 6 | Clopton Middle School | A | Truetken | Simmons | Gronek | Mitchell | 5:38.86 | Verified |
| 7 | Wright City MS | A | Juden | Mikus | Walch | Ward | NT | Unverified: |
