| PL | Athlete | Yr. | Team | Time | Pts | HPL |
|---|---|---|---|---|---|---|
| 1 | KECK, Montgomery | SO | Dixon High School | 28.93 | 10 | 3 (1) |
| 2 | TODD, Kelsey | SR | Crocker High School | 29.84 | 8 | 1 (1) |
| 3 | BUTLER, Madeline | SR | Dixon High School | 30.29 | 6 | 3 (2) |
| 4 | EDGAR, Erica | 9 | Steelville High School | 30.36 | 5 | 3 (3) |
| 5 | BARKER, Alexus | SR | Viburnum High School | 30.38 | 4 | 3 (4) |
| 6 | VOGELSANG, BROOKELYN | 9 | New Haven High School | 30.52 | 3 | 2 (1) |
| 7 | SETZER, Navy | JR | St. James High School | 30.98 | 2 | 2 (2) |
| 8 | DONG, Justina | SR | Cuba High School | 31.00 | 1 | 3 (5) |
| 9 | PERKINS, Marli | SO | Steelville High School | 31.12 | 2 (3) | |
| 10 | HARRIS, Kaliethia | JR | Newburg High School | 31.16 | 3 (6) | |
| 11 | DEARDEUFF, Erika | JR | St. James High School | 31.60 | 2 (4) | |
| 12 | GRATER, Chloe | SR | New Haven High School | 32.45 | 3 (7) | |
| 13 | HALBERT, Natalie | 9 | Viburnum High School | 33.28 | 2 (5) | |
| 14 | BLACKMON, Elissa | SO | St. Paul Lutheran (Farmington) | 33.32 | 1 (2) | |
| 15 | SASFY, Jordan | 9 | Crocker High School | 33.45 | 2 (6) | |
| 16 | ROSENTRETER, Riley | JR | Vienna High School | 33.74 | 2 (7) | |
| 17 | PRATT, Ciarra | SO | Newburg High School | 33.91 | 3 (8) | |
| 18 | BAER, Anna | 9 | St. Paul Lutheran (Farmington) | 36.85 | 1 (3) | |
| 19 | SOPPELAND, Tanya | 9 | Cuba High School | 37.83 | 1 (4) | |
| 20 | GRIFFIN, Aliyah | SO | Vienna High School | 38.88 | 2 (8) |
