| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Miller Career Academy | A | Ammons | Brown | Harris | RUTLIN | NT | Unverified: |
| 2 | Lutheran South High School | A | Saitz | Hayes | Karsten | Allen | NT | Unverified: |
| 3 | St. Louis Blue Knights | A | Ward | Snell | Hughes | Bryant | NT | Verified |
| 4 | John Burroughs High School | A | 53.50 | Unverified: | ||||
| 5 | MICDS | A | Harrell | Ferguson | Brothers | Zar | 55.51 | Verified |
| 6 | Principia High School | A | Gordon | McKim | Parker | Turnbaugh | NT | Unverified: |
| 7 | Clayton High School | A | Hicks | Hopper | Stern | Sanders | NT | Unverified: |
