| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | MICDS | A | McCarthy | Helms | Hendricks | Strelo | 13:03.78 | Unverified: |
| 2 | University City High School | A | Klein | Holland | Bostic | Trobaugh | NT | Unverified: |
| 3 | Lutheran South High School | A | Hickerson | Meier | Spear | Burgdorf | NT | Unverified: |
| 4 | Principia High School | A | Heath | Omware | Nutwell | Asinga | NT | Unverified: |
| 5 | Clayton High School | A | Cohn | Lenze | Smith | Saner | DQ | Unverified: |
| 6 | St. Louis Blue Knights | A | Gresham | Meyers | Hillebrand | Snell | NT | Unverified: |
