| PL | # | Athlete | Yr. | Team | Time | Pts | HPL |
|---|---|---|---|---|---|---|---|
| 1 | 126 | Sanaa WASHINGTON | FR | Bryan Station | 26.02 | 10 | 1 (1) |
| 2 | 269 | Naturi ROBINSON | SR | Tates Creek | 26.93 | 8 | 1 (2) |
| 3 | 131 | Simone BESSONG | 8 | Cooper | 26.99 | 6 | 1 (3) |
| 4 | 173 | Kendra DEROY | SR | Henry Clay | 28.08 | 4 | 1 (4) |
| 5 | 186 | Ainsley WILSON | SO | Henry Clay | 28.65 | 2 | 3 (1) |
| 6 | 252 | Alexis HENRY | SR | Sayre | 28.99 | 1 | 1 (5) |
| 7 | 261 | Maquoia RAGLIN | JR | Scott County | 29.05 | 1 (6) | |
| 8 | 203 | Sophee OPALKA | SO | Lexington Catholic | 29.25 | 4 (1) | |
| 9 | 164 | Allyson NOWLIN | FR | Great Crossing | 29.26 | 1 (7) | |
| 10 | 161 | Elise EDISON | SO | Great Crossing | 29.50 | 2 (1) | |
| 11 | 215 | Olivia GRIESE | JR | Lexington Christian | 29.58 | 4 (2) | |
| 12 | 123 | Victory SLEDGE | FR | Bryan Station | 29.65 | 3 (2) | |
| 13 | 260 | Lacy MURPHY | SR | Scott County | 29.75 | 1 (8) | |
| 14 | 282 | Chloe BROWN | FR | Woodford County | 30.26 | 2 (2) | |
| 15 | 267 | Brianna DOWELL | SR | Tates Creek | 30.41 | 2 (3) | |
| 16 | 154 | Maria TAPIA | FR | Frederick Douglass | 31.49 | 2 (4) | |
| 17 | 244 | Jakyliah KENNEDY | FR | Paul Dunbar | 31.59 | 3 (3) | |
| 18 | 212 | Sydney FISCHER | SO | Lexington Christian | 31.83 | 4 (3) | |
| 19 | 207 | Katie SEITZ | SO | Lexington Catholic | 31.85 | 4 (4) | |
| 20 | 284 | Bailey FOUTS | FR | Woodford County | 31.87 | 2 (5) | |
| 21 | 101 | Alyssa BROOKS | JR | Bell County | 32.09 | 3 (4) | |
| 22 | 153 | Jasmine STARKS | SO | Frederick Douglass | 32.10 | 2 (6) | |
| 23 | 238 | Livi COSTES | JR | Paul Dunbar | 32.53 | 4 (5) | |
| 24 | 102 | Jasmine CLOUSE | SO | Bell County | 33.16 | 3 (5) | |
| 25 | 228 | Ann MALCOMB | FR | Owen County | 34.10 | 2 (7) | |
| 26 | 225 | Gracie BRUENER | JR | Owen County | 34.13 | 3 (6) | |
| 27 | 275 | Delaney JOHNSON | SO | Trinity Christian Academy | 36.65 | 2 (8) | |
| 28 | 114 | Gabriella WEISS | SR | Bluegrass United Home School Tea | 38.81 | 4 (6) |