| PL | # | Athlete | Yr. | Team | Time | Pts | HPL |
|---|---|---|---|---|---|---|---|
| 1 | 722 | Alexander BOYD | JR | Seneca | 52.05 | 10 | 1 (1) |
| 2 | 748 | Jackson GORDON | JR | Woodford County | 52.58 | 8 | 1 (2) |
| 3 | 582 | Kerrington EUBANKS | JR | Frederick Douglass | 53.19 | 6 | 1 (3) |
| 4 | 552 | Austin LIVERSGOWDEY | SO | Cooper | 53.50 | 4 | 1 (4) |
| 5 | 645 | Andrew FINLEY | JR | Lexington Catholic | 54.90 | 2 | 2 (1) |
| 6 | 658 | Evan BROWN | SO | Lexington Christian | 55.42 | 1 | 3 (1) |
| 7 | 538 | Isack KIPANGA | SR | Bryan Station | 55.51 | 1 (5) | |
| 8 | 650 | Xavier HENDERSON | JR | Lexington Catholic | 56.89 | 3 (2) | |
| 9 | 746 | Fabian GARCIA | JR | Woodford County | 57.33 | 3 (3) | |
| 10 | 683 | Ronan BAVISHI | FR | Paul Dunbar | 57.50 | 2 (2) | |
| 11 | 680 | Brandon SNELL | SO | Owen County | 57.61 | 2 (3) | |
| 12 | 703 | Marcum MCLEAN | SR | Sayre | 57.96 | 2 (4) | |
| 13 | 563 | Preston WINIGER | FR | Cooper | 58.01 | 1 (6) | |
| 14 | 696 | Delane SMITH | FR | Paul Dunbar | 58.05 | 1 (7) | |
| 15 | 662 | Aiden FINLEY | FR | Lexington Christian | 59.58 | 2 (5) | |
| 16 | 717 | Jacob OLSEN | JR | Scott County | 1:00.28 | 3 (4) | |
| 17 | 711 | Danny ARMSTRONG | SO | Scott County | 1:00.46 | 3 (5) | |
| 18 | 637 | Reed RICHARDSON | SO | Lafayette | 1:00.87 | 3 (6) | |
| 19 | 705 | Carlos RAMIREZ | SR | Sayre | 1:01.45 | 2 (6) | |
| 20 | 601 | William BARRETT | SO | Great Crossing | 1:02.27 | 2 (7) | |
| 21 | 607 | Bryce LEE | SO | Great Crossing | 1:02.34 | 2 (8) | |
| 22 | 526 | Jose BRAVO | SO | Bryan Station | 1:02.39 | 1 (8) | |
| 23 | 516 | Topher BRADLEY | 8 | Bluegrass United Home School Tea | 1:04.85 | 3 (7) | |
| 24 | 520 | RJ TODD | FR | Bluegrass United Home School Tea | 1:08.41 | 3 (8) |