| !! | Meet Record | 24.96 | Jazzmin Jeter | Ballard | 
| PL | # | Athlete | Yr. | Team | Time | Pts | HPL | Note | 
|---|---|---|---|---|---|---|---|---|
| 1 | 35 | Daneezia PATTON | JR | Boyle County | 26.17 | 10 | 1 (1) | |
| 2 | 158 | Alexis PINKSTON | JR | Henry Clay | 26.53 | 8 | 1 (2) | |
| 3 | 194 | Rosa SMITH | 9 | Lafayette | 27.33 | 6 | 2 (1) | |
| 4 | 270 | Savannah LEWIS | SO | Oldham County | 27.61 | 4 | 2 (2) | |
| 5 | 88 | Elizabeth FRANKLIN | JR | Frederick Douglass | 27.85 | 2 | 1 (3) | |
| 6 | 150 | Kendra DEROY | SO | Henry Clay | 28.11 | 1 | 2 (3) | |
| 7 | 266 | Haedin GOLDSMITH | JR | Oldham County | 28.15 | 1 (4) | ||
| 8 | 192 | Kailynn PARIS | 9 | Lafayette | 28.44 | 2 (4) | ||
| 9 | 330 | Bailie COLCORD | SR | Scott County | 28.72 | 2 (5) | ||
| 10 | 65 | Amaya HIGGINS | SO | East Jessamine | 28.91 | 3 (1) | ||
| 11 | 240 | Dani REINHARDT | 7 | Madison Central | 29.05 | 5 (1) | ||
| 12 | 197 | Alexis BROCK | JR | Lexington Catholic | 29.12 | 2 (6) | ||
| 13 | 82 | Deja ALLISON | SO | Frederick Douglass | 29.13 | 2 (7) | ||
| 14 | 254 | Elizabeth PREWITT | JR | Madison Southern | 29.18 | 3 (2) | ||
| 15 | 304 | Jaia MORRIS | JR | Quest Charter | 29.27 | 3 (3) | ||
| 16 | 297 | Jodell MASSAQUOI | SO | Paul Dunbar | 29.48 | 3 (4) | 29.472 | |
| 17 | 374 | Caroline ABSHER | JR | West Jessamine | 29.48 | 2 (8) | 29.476 | |
| 18 | 78 | Jaydan WALLACE | SO | Franklin County | 29.51 | 4 (1) | ||
| 19 | 204 | Katelyn LENNON | JR | Lexington Catholic | 29.81 | 3 (5) | ||
| 20 | 249 | Ashley COLLINS | SO | Madison Southern | 29.89 | 4 (2) | ||
| 21 | 333 | Allison FAIRCLOTH | JR | Scott County | 30.00 | 3 (6) | ||
| 22 | 173 | MacKenzie FOUNTAIN | 9 | Jeffersonville High School | 30.01 | 1 (5) | ||
| 23 | 321 | Julianna CLAYTON | 9 | Sayre | 30.02 | 4 (3) | ||
| 24 | 81 | Kennedy YAGEL | 9 | Franklin County | 30.16 | 1 (6) | ||
| 25 | 407 | Madison PEACE | 7 | Williamsburg | 30.30 | 4 (4) | ||
| 26 | 435 | Asia SHANDS | SO | Woodford County | 30.60 | 3 (7) | ||
| 27 | 241 | Mari REINHARDT | 7 | Madison Central | 31.04 | 4 (5) | ||
| 28 | 132 | Alissa PASLEY | 8 | George Rogers Clark | 31.94 | 5 (2) | ||
| 29 | 123 | Amelia JONES | 7 | George Rogers Clark | 32.63 | 4 (6) | ||
| 30 | 289 | Asha GILL | 9 | Paul Dunbar | 32.97 | 5 (3) | ||
| 31 | 15 | Ajiyah HARISTON | SO | Bell County | 33.24 | 5 (4) | ||
| 32 | 426 | Rayjanna HOWARD | 9 | Woodford County | 33.26 | 3 (8) | ||
| 33 | 222 | Macy MEYER | 9 | Lexington Christian | 33.42 | 1 (7) | ||
| 34 | 957 | Brooklyn CHRISTOPHER | 7 | George Rogers Clark | 33.52 | 4 (7) | 33.511 | |
| 35 | 206 | Macy POWELL | SO | Lexington Catholic | 33.52 | 5 (5) | 33.512 | |
| 36 | 136 | Hannah RUNYON | 8 | George Rogers Clark | 33.55 | 5 (6) | ||
| 37 | 403 | Alana MAH | 8 | Williamsburg | 33.78 | 4 (8) | ||
| 221 | Ava MEYER | 8 | Lexington Christian | NT |