| !! | Meet Record | 4:07.02 | G Blair, K Briscoe, T Chism, A LaPrelle | Henry Clay | 
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note | |
|---|---|---|---|---|---|---|---|
| 1 | Rockcastle County | A | Dotson, Victoria | Dotson, Alexis | Martin, Allie | Watson, Emma | Unverified: | 
| 2 | Oldham County | A | Davis, Caroline | Chapman, Katy | Thomas, Ana | Despain, Josie | Verified | 
| 3 | Boyle County | A | Bugg, Payton | Kirkland, Kennedy | Jackson, Emma | Lanigan, Kaylee | Unverified: | 
| 4 | Jeffersonville High School | A | Cofie, Tara | Bates, Navaeh | Gibson, Bailey | Fountain, MacKenzie | Unverified: | 
| 5 | Woodford County | A | Bradshaw, Esther | Jackson, Adriana | Gormley, Callie | Bleuel, Abbey | Verified | 
| 6 | West Jessamine | A | Absher, Caroline | Dunlap, Rebecca | Gouge, Shannon | Hawley, Madi | Unverified: | 
| 7 | Frederick Douglass | A | Allison, Deja | Clark, Tiana | Franklin, Elizabeth | El-Amin, Amina | Unverified: | 
| 8 | Corbin | A | Steely, Lauren | Faulkner, Lauren | Helton, Jill | Simons, Lauren | Unverified: | 
| 9 | Williamsburg | A | Mattingly, Lylah | Peace, Madison | Abbott, Grace Ann | Mattingly, Selena | Verified | 
| 10 | Lexington Catholic | A | Robinson, Bronwyn | Powell, Macy | Lennon, Katelyn | Rowe, Abbey | Verified | 
| 11 | Madison Southern | A | Prewitt, Elizabeth | Stanton, Katilyn | Stanton, Summer | Collins, Ashley | Verified | 
| 12 | Garrard County | A | Cortes, Marianna | Hale, Lexi | Lawrence, Emma | Morrow, Molly | Unverified: | 
| 13 | George Rogers Clark | A | Hughbanks, Veronika | Taylor, Emma | Jackson, Maria | Mitmesser, Ellie | Unverified: | 
| 14 | Trinity Christian Academy | A | Walden, Megan | Rowe, Kendall | Kitchens, Ella | Randle, Sarah | Unverified: Lafayette All Comers | 
| 15 | Lexington Christian | A | Colon, Sydney | Hayes, Kendall | Meyer, Ava | Spillman, Sydney | Unverified: | 
| 16 | Paul Dunbar | A | Joshi, Gia | Joshi, Isha | Gill, Asha | Baird, Brittany | Unverified: | 
| 17 | Franklin County | A | Unverified: | ||||
| 18 | Sayre | A | Henry, Alison | Whitaker, Lindsay | Patterson, Elizabeth | Frasca, Athena | Unverified: |