| !! | Meet Record | 42.68 | Henry Clay | 
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note | |
|---|---|---|---|---|---|---|---|
| 1 | Trinity (Louisville) | A | Feger, Kyle | Moore, Elijah | Miller, Ryan | Forehand, Chris | Unverified: | 
| 2 | Trinity (Louisville) | b | Montgomery, Austin | Forehand, Chris | Johnson, Wynton | Strickland, Roderick | Verified | 
| 3 | Henry Clay | A | Jackson, Langston | Jackson, Ramond | Neal, Kobie | Crutchfield, Ari | Unverified: | 
| 4 | Frederick Douglass | A | Lewis, Christian | White, Deiron | Roberts-Hasson, Amir | Russell, Jaylin | Unverified: | 
| 5 | East Jessamine | A | Verified | ||||
| 6 | George Rogers Clark | A | Lin, Tony | Conner, A.J. | Wilson, Caleb | Israel, Azariah | Unverified: | 
| 7 | George Rogers Clark | b | Mann, Angus | Jones, Kobe | Chea, Cobe | Webb, Elias | Verified | 
| 8 | Corbin | A | Longmire, Treyveon | Johnson, Taylor | Shelton, Cole | Skinner, Brodie | Unverified: | 
| 9 | Franklin County | A | Verified | ||||
| 10 | Williamsburg | A | Davis, Kyle | Thomas, Gavon | Rainwater, Jayden | Mattingly, Eli | Unverified: | 
| 11 | Madison Central | A | Rogers, Alex | Garner, Jashe | Hulberg, Ashton | Davis, Isaiah | Verified | 
| 12 | Lexington Catholic | A | Gallagher, Ryan | Gallagher, Sean | Henderson, Patrick | Luna-Garcia, Kevin | Verified | 
| 13 | Lafayette | A | Sanders, Ta | Stone, Elijah | Morrison, Diante | Toure, Oumar | Unverified: | 
| 14 | Bell County | A | Dennis, Ashlund | Bailey, Hunter | Jones, Jason | Stephney, London | Verified | 
| 15 | Madison Southern | A | Jones, AJ | Negron, Joseph | Tinsley, Clay | Feltner-Reichert, Noel | Verified | 
| 16 | Paul Dunbar | A | Drew, Shyhiem | Ilunga, Frank | Pascal, Andre | Hamilton, Jack | Verified | 
| 17 | West Jessamine | A | Dunagan, Matthew | Fitzpatrick, Caleb | Tackett, Micah | Swentzel, Jamesy | Verified | 
| 18 | Woodford County | A | Foley, Gabreal | O'Toole, Nick | Alexander, Jahdon | Raglin, Corey | Verified | 
| 19 | Lexington Christian | A | Brand, Jacob | Duerler, Collin | Hinton, Josh | Strunk, Josh | Unverified: | 
| 20 | Scott County | A | Griggs, Caleb | Hamiliton, Jeremy | Hearn, Bryce | Emongo, Silas | Unverified: |