| !! | Meet Record | 48.12 | Tates Creek | 
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note | |
|---|---|---|---|---|---|---|---|
| 1 | Tates Creek | A | Davidson, Myann | Hollingsworth, Rosalynd | Robinson, Naturi | Smalley, Kayla | Verified | 
| 2 | Jeffersonville High School | A | Baker, Ahmya | Todd, Tamia | Winford, Aa Leiah | Tate, Callista | Verified | 
| 3 | Oldham County | A | Goldsmith, Haedin | Lewis, Savannah | Davis, Caroline | Despain, Josie | Verified | 
| 4 | Frederick Douglass | A | Allison, Deja | McGuffey, Alaysia | Suarez, Ana | Clark, Tiana | Verified | 
| 5 | East Jessamine | A | Holcomb, Ma'Lonn | Mckinney, Jada | Campbell, Jameka | Higgins, Amaya | Verified | 
| 6 | Madison Central | A | Mason, Keyandra | Todd, Taleya | Winn, Tajanna | Reinhardt, Mari | Verified | 
| 7 | Henry Clay | A | DeRoy, Kendra | Pinkston, Alexis | Gatewood, Jaden | Cantrell, Angel | Unverified: | 
| 8 | Franklin County | A | Yagel, Kennedy | Wallace, Jaydan | Combs, Jayde | Wiser, Justice | Verified | 
| 9 | Madison Southern | A | Gilliam, Rebekah | Prewitt, Elizabeth | Hisle, Hannah | VanDeCar, Rylee | Verified | 
| 10 | West Jessamine | A | Absher, Caroline | Jatau, Shalomel | Jatau, Sophie | Teater, Ashleigh | Unverified: | 
| 11 | Woodford County | A | Shands, Asia | Ruvalcaba, Ana | Jackson, Adriana | Howard, Rayjanna | Verified | 
| 12 | Corbin | A | Steely, Lauren | Jones, Payton | Russell, Makenna | Estep, Cady | Unverified: | 
| 13 | Rockcastle County | A | Bullen, Destiny | Burdette, Abby | Delph, Darla | Loudermilk, Karalinn | Verified | 
| 14 | Williamsburg | A | Dople, Jenna | Chapman, Ashley | Taylor, Madison | Moses, Jamie | Verified | 
| 15 | George Rogers Clark | A | Southwood, Emily | Shelton, Carly | Pendergraft, Taylor | Kegley, Mackenzie | Unverified: | 
| 16 | Lexington Catholic | A | Regard, Kate | Owsley, Sierra | Duffey, Olivia | Smith, Cassidy | Unverified: | 
| 17 | Bell County | A | Hariston, Ajiyah | Brooks, Alyssa | Partin, Victoria | Miracle, Lenia | Unverified: | 
| 18 | Lexington Christian | A | Casper, Gretchen | Colon, Sydney | Crager, Holynn | Harper, Sarah | Unverified: | 
| 19 | Paul Dunbar | A | Dabit, Layla | Hinshaw, Carly | Joshi, Isha | Joshi, Gia | Unverified: | 
| 20 | Scott County | A | Varney, MaKayla | Shepherd, Lexie | Shepherd, Riley | Allen, Kiya | Unverified: |