| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | Olathe Heritage Christian | A | 1:04.00 |
| 2 | Summit Christian Academy MS | A | 58.57 |
| 3 | Barstow Middle School | A | 54.68 |
| 4 | University Academy Charter MS | A | 47.10 |
| 5 | Pembroke Hill School | A | 48.90 |
| 6 | Kansas City Christian Middle Sch | A | 58.45 |
| 7 | Maranatha Academy Middle School | A | 59.82 |
| 8 | Bishop Seabury Academy Middle Sc | A | NT |