| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 |
|---|---|---|---|---|---|---|
| 2 | St. Elizabeth | A | Heits | Brewster | Courter | Stegall |
| 3 | Presentation Middle School | A | Allen | Bahr | Ryan | Jacobs |
| 4 | Visitation | A | O'Laughlin | Archer | Ketchum | Fendler |
| 5 | Notre Dame de Sion Middle School | A | Carnegie | Johnson | Martens | Milligan |
| 6 | Oak Hill Middle School | A | Chen | Haar | Wallace | Phung |
| 7 | St. Elizabeth | B | Pace |