| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | NAGY, Angie | Unattached | 5:27.00 | |
| 2 | BERRY, Faith | Unattached | 5:30.00 | |
| 3 | ERDMANN, Monica | Unattached | 5:34.00 | |
| 4 | ERICKSON, Hannah | Unattached | 5:40.00 | |
| 5 | KERN, Li Anne | Unattached | 5:50.00 | |
| 6 | DEINES, Jessica | Unattached | 5:50.90 | |
| 7 | STOVALL, Anna | Unattached | 6:00.00 | |
| 8 | WATKINS, Karen | Unattached | 6:03.18 | |
| 9 | MACMANN, Katy | Unattached | 6:03.78 | |
| 10 | BICKLING, Mackenzie | Unattached | 6:04.00 | |
| 11 | BOJARSKI-STAUFFER, Alexandra | Unattached | 6:15.00 | |
| 12 | SHERER, Britt | Unattached | 6:20.00 | |
| 13 | KAVANAGH, Kathleen | Unattached | 6:22.00 | |
| 14 | WHEELER, Shelia | Unattached | 6:22.90 | |
| 15 | FACKRELL, Lily | Unattached | 6:30.00 | |
| 16 | MOTTET, Cameron | Unattached | NT | |
| 17 | BADER, Stevie | Unattached | NT | |
| 18 | BILHIMER, Sarah | Unattached | NT |