| Lane | Team | Squad | Note |
|---|---|---|---|
| 3 | Oskaloosa High School | A | Verified |
| 4 | Valley Falls High School | A | Verified |
| 5 | Horton High School | A | Verified |
| Lane | Team | Squad | Note |
|---|---|---|---|
| 1 | Jackson Heights High School | A | Verified |
| 2 | Mission Valley High School | A | Verified |
| 3 | Valley Heights High School | A | Unverified: Twin Valley League Meet |
| 4 | Wabaunsee High School | A | Verified |
| 5 | Doniphan West High School | A | Unverified: 2025 TVL Meet |
| 6 | Jefferson County North High Scho | A | Verified |
| 7 | Maranatha Christian Academy | A | Verified |