| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PUCHALLA, Gavin | 9 | St. James Academy | NT | Unverified: |
| 2 | BRUGGEMAN, Brody | 9 | St. James Academy | NT | Unverified: |
| 3 | ROSS, Malachi | JR | Grandview High School | 49.09 | Verified |
| 4 | JOHNSON, Cobi | JR | Grandview High School | 42.00 | Unverified: Grandview Time Trial |
| 5 | WRIGHT, Calan | SR | Grandview High School | 45.00 | Unverified: Grandview Time Trial |
| 6 | BALL, Dylan | SO | Fort Osage High School | NT | Unverified: |
| 7 | LAUAKI, Gordon | 9 | Fort Osage High School | NT | Unverified: |
| 8 | RYNE, Cooper | 9 | St. James Academy | NT | Unverified: |