| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 6 | Madelyn BERGLUND | XLR8 IOWA TRACK CLUB | 2:35.00 | ||
| 7 | Alyssa RICHMOND | 16 | XLR8 IOWA TRACK CLUB | 2:35.00 | |
| 8 | Brecklyn PETERSEN | 15 | XLR8 IOWA TRACK CLUB | 2:40.00 | |
| 9 | Briana EDWARDS | 15 | 28-UNATTACHED | 2:41.00 | |
| 10 | Hayley CONNER | 16 | UNATTACHED | 3:01.03 |