| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Ny'Asia THOMAS | 16 | APOLLO TRACK CLUB | NT | |
| 2 | Emma RAMBOLDT | 15 | HOT FEET TRACK CLUB | 1:03.00 | |
| 3 | Briana EDWARDS | 15 | 28-UNATTACHED | 1:06.00 |
| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Ally HUXTABLE | 16 | 27-UNATTACHED | 1:02.00 | |
| 2 | Delia GREGORY | 16 | HOT FEET TRACK CLUB | 1:00.00 | |
| 3 | Jazzmyne MEANS | 16 | KC FLYERS TRACK CLUB | 1:00.89 |