| Lane | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Jaci SIEVERS | 18 | UNATTACHED | 4:51.00 | |
| 2 | Lida PADGETT | 17 | 28-UNATTACHED | 5:21.00 | |
| 3 | Mayson HARTLEY | 17 | XLR8 IOWA TRACK CLUB | 5:25.00 | |
| 4 | Lilly MAUK | 17 | UNATTACHED | 5:38.00 | |
| 5 | Lauren BISHARD | 17 | 28-UNATTACHED | 5:45.00 | |
| 6 | Jessa CASSITY | 17 | UNATTACHED | 5:57.00 | |
| 7 | Ella THIER | 18 | UNATTACHED | NT |