| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | FRAZIER, Ty'Anna | Whiteland Community | 53.99 |
| 2 | NASH, Taylor | New Palestine | 53.23 |
| 3 | SCHWEIGERT, Cameron | West Lafayette | 52.35 |
| 4 | MUNTU, Queren | Avon | 51.26 |
| 5 | PFISTER, Maddy | Pendleton Heights | 51.15 |
| 7 | STONE, Mary | Zionsville | 51.83 |
| 8 | ERICKSON, Elle | Zionsville | 52.45 |
| 9 | GUSTUS, Addie | West Lafayette | 53.34 |
| 10 | DORAN, Alaina | New Palestine | 54.30 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | SMITH, Cierra | Brownsburg | 50.44 |
| 3 | MOSER, Ruth | Harrison (West Lafayette) | 47.39 |
| 4 | N'GBESSO, Sophia | Brownsburg | 46.35 |
| 5 | KUBACKI, Kate | Indianapolis Cathedral | 44.13 |
| 6 | HAUSER, Lucy | Westfield | 45.40 |
| 7 | AHNERT, Elsie | Harrison (West Lafayette) | 47.36 |
| 8 | SMITH, Jackie | Floyd Central | 47.99 |
| 9 | LINVILLE, Lauren | Westfield | 50.18 |
| 10 | SMITH, Stephanie | Floyd Central | 50.58 |