8:10PM
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | O'SHEA, Ciara | Madison Central | 4:57.17 | |
| 2 | SECOR, Jessica | DuPont Manual | 4:59.55 | |
| 3 | BEITING, Caroline | Lex. Catholic | 5:03.50 | |
| 4 | FAWBUSH, Tula | Oldham County | 5:06.12 | |
| 5 | RAGLAND, Reese | Louisville Colle | 5:07.42 | |
| 6 | GILMORE, Reagan | Assumption | 5:09.44 | |
| 7 | HOLBROOK, Olivia | Campbell County | 5:10.83 | |
| 8 | DOTSON, Lexi | Rockcastle Co. | 5:11.63 | |
| 9 | LIDDLE, Emily | Bluegrass United | 5:12.41 | |
| 10 | HERNANDEZ, Kylie | Bourbon County | 5:13.61 | |
| 11 | KOPSER, Allison | Ryle | 5:13.91 | |
| 12 | STRONG, Maddie | Scott County | 5:13.94 | |
| 13 | SCHMITT, Julia | Assumption | 5:16.88 | |
| 14 | MCPHERSON, Chesney | Barren County | 5:17.25 | |
| 15 | SHELTON, Audrey | Grant County | 5:17.67 | |
| 16 | OVERBAY, Abby | South Warren | 5:17.70 |