| $ | Stadium | 52.01 | Eric Bologa | Shippensburg | 2015 |
| $ | Stadium | 52.01 | Bruce Tiller | Kutztown | 1985 |
| $ | Stadium | 52.01 | Jack Goldowski | ESU | 1985 |
| N | Meet | 53.09 | Ronald D'Eliseo | ESU |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | Bradley Spencer | Wilmington (Del.) |
59.52 | Towson Invitational 2022 |
| 3 | Nasir Kyle | East Stroudsburg |
58.37 | 39th Millersville Metrics |
| 4 | Eric Anderson | Molloy |
56.71 | Ursinus College Outdoor Invitational |
| 5 | Shemar Parker | Wilmington (Del.) |
57.10 | Towson Invitational 2022 |
| 6 | Billy Wren | Molloy |
58.77 | ECC Outdoor Track and Field Championships |
| 7 | Makendy Midouin | St. Thomas Aquinas |
59.68 | 2019 ECC Outdoor Championships |
| 8 | Matt Linbrunner | East Stroudsburg |
1:00.10 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | Caleb Carney | East Stroudsburg |
1:01.00 | |
| 3 | Daniel Daley | East Stroudsburg |
1:01.00 | |
| 4 | Don Hill | Kutztown |
1:00.30 | |
| 5 | Shervin Mokhtari | Scranton |
1:00.69 | 2022 Messiah Multi/Invitational |
| 6 | Eric Krzyzaniak | East Stroudsburg |
1:01.00 | |
| 7 | Liam Beifus | East Stroudsburg |
1:01.16 | 39th Millersville Metrics |
| 8 | Alex Quillan | St. Thomas Aquinas |
1:01.55 | Misericordia Twilight |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | Thomas Bovenberg | St. Thomas Aquinas |
1:06.39 | Misericordia Twilight |
| 3 | Zandrew Bowe | East Stroudsburg |
1:04.09 | 39th Millersville Metrics |
| 4 | Stephane Fortune | St. Thomas Aquinas |
1:01.97 | Misericordia Twilight |
| 5 | Thomas Albright | Penn State T&F Club | 1:04.00 | |
| 6 | Bladimir Pena | SUNY Delhi |
1:04.46 | Inter-Conference Championship Meet |
| 7 | Caleb Diaz Viola | East Stroudsburg |
1:06.41 | 39th Millersville Metrics |
| 8 | Brendon Arcara | Scranton |
1:06.77 | Muhlenberg Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | Kuron Williams | Lehman |
1:10.42 | Peacocks and Brick City Classic 19 |
| 4 | Mickenson Simon | SUNY Delhi |
1:07.38 | Utica Pioneer Spring Invite |
| 5 | Obinna Njoku II | Lehman |
1:09.93 | Vassar College April Invitational |