| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | KAISER, Abigail | Trinity HS-S | 2:28.00 |
| 2 | VALASEK, McKenna | Faith WV HS-S | 2:36.50 |
| 3 | HUYARD, Mariana | Dayspring HS-L | 2:43.97 |
| 4 | GOOD, Jocelyn | Lancaster HS-L | 2:46.40 |
| 5 | CASTRONOVO, Olivia | Calvary HS-L | 2:49.70 |
| 6 | MCCARTHY, Joyanne | Lancaster HS-L | 3:01.00 |
| 7 | RITTENHOUSE, Jules | Lancaster HS-L | 3:19.14 |
| 8 | EROH, Eva | Immanuel HS-S | 3:33.00 |
| 9 | BIALECKI, Hazlee | Columbia HS-S | NT |
| 10 | PEREIRA, Mariana | Abundant HS-L Life | NT |