| N | Meet | 2:30.01 | Stafford McFarlane | Essex | 2008 |
| # | Facility | 2:30.01 | Stafford McFarlane | Essex | 2008 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Luke Valdevit | East Stroudsburg |
2:30.00 | |
| 2 | khabeeba Mills | Essex CC | 2:32.70 | Villanova Invitational |
| 3 | Alexander Fillman | Franklin & Marshall |
2:33.00 | |
| 4 | Ryan Braun | Franklin & Marshall |
2:33.00 | |
| 5 | Thomas Perrone | Pratt Institute | 2:40.52 | Bill Ward Invitational |
| 6 | Cameren Brice | Keystone |
2:43.40 | Crowell Open 2019 |
| 7 | Daniel McDonald-Hylton | Essex CC | 2:48.06 | Villanova Invitational |
| 8 | Cody Davis | Sacred Heart |
2:51.25 | |
| 9 | Bruce Westgate | Pratt Institute | 2:54.84 | Bill Ward Invitational |
| 10 | Logan Reppard | Broome | 3:20.00 | |
| 11 | Davian Gray | Essex CC | NT | |
| 12 | Tim Connell | DeSales |
NT | |
| 13 | Corey Scochin | Keystone |
NT |