| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | 890 | BERTOIA, Ava | Royal City Track & Field Clu | 5:01.00 | |
| 2 | 866 | KENNEDY, Katherine | Kajaks Track and Field | 4:50.91 | |
| 3 | 859 | KOONER, Sohanna | Kajacks | 4:52.00 | |
| 4 | 985 | DUFF, Hadassah | Trinity Western | 4:48.00 | |
| 5 | 154 | BALL, Zoe | Vancouver Thunderbirds | 5:03.65 | |
| 6 | 939 | WATKINS, Rachael | Simon Fraser |
4:31.90 | 2025 GNAC Outdoor TF Championships |
| 7 | 930 | DEE, Veronica | Simon Fraser |
4:34.50 | |
| 8 | 992 | RODWAY, Ava | Trinity Western | 4:46.22 | |
| 9 | 157 | SCHULZ, Emmy | Vancouver Thunderbirds | 5:15.91 | |
| 10 | 931 | KALISCH, Milena | Simon Fraser |
4:45.00 | |
| 11 | 172 | TASDEMIR, Selin | Simon Fraser |
4:34.00 | |
| 12 | 993 | ROPER, Catherine | Trinity Western | NT |