| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | 428 | WITHERS, Naomi | Royal City Track & Field Club | 50.60 | |
| 2 | 353 | GAVIN, Kensie | Ocean Athletics Track & Field Club | 45.13 | |
| 3 | 112 | RUTHERFORD, Paige | Golden Ears Athletics | NT | |
| 4 | 130 | HOPWOOD, Victoria | Kajaks Track & Field Club | NT | |
| 5 | 108 | NAPIER, Skye | Golden Ears Athletics | NT | |
| 6 | 427 | TRIEU, Ronnie | Royal City Track & Field Club | 50.08 | |
| 7 | 620 | CHENE, Jocelyne | Vancouver Thunderbirds | 47.23 | |
| 8 | 214 | CHUNG, Jasmine | Langley Mustangs | NT |