| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Custer County High School | A | Stover | Richardson | Ruth | Bachman | NT |
| 3 | South Park High School | A | 4:12.81 | ||||
| 4 | Buena Vista High School | A | Schuknecht | Whitmore | Reed | Montoya | 3:53.45 |
| 5 | Buena Vista High School | B | Flowers | Torrens | Book | Schuknecht | DNS |