| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | Musk, Angela | SR | Bellarmine | 4:59.77 |
| 2 | Sebby, Krysten | JR | Lewis | 5:09.31 |
| 3 | Steenstrup, Kelsey | FR | Wis.-Parkside | 5:09.82 |
| 4 | Wagner, Maggie | SR | Bellarmine | 5:09.95 |
| 5 | Mornout, Jordan | JR | Southern Indiana | 5:10.04 |
| 6 | Knapp, Sutton | JR | Indianapolis | 5:10.19 |
| 7 | Zittleman, Amanda | SO | Wis.-Parkside | 5:10.92 |
| 8 | Miller, Taylor | JR | Wis.-Parkside | 5:11.38 |
| 9 | Kiblinger, Elizabeth | FR | Maryville (Mo.) | 5:12.60 |
| 10 | Hahn, Monica | FR | Lewis | 5:14.96 |
| 11 | Frith, Emily | SO | Bellarmine | 5:15.12 |
| 12 | Senn, Rebecca | JR | Drury | 5:22.79 |
| 13 | Dickerson, Taylor | FR | Maryville (Mo.) | 5:23.67 |
| 14 | Clawson, Amy | JR | Lewis | 5:24.36 |
| 15 | Smith, Alyson | SO | Missouri S&T | 5:24.48 |
| 16 | Wittig, Haley | JR | Indianapolis | 5:24.87 |
| 17 | Hanlon, Trisha | JR | St. Joseph's (Ind.) | 5:25.04 |
| 18 | Simpson, Sheena | JR | Maryville (Mo.) | 5:25.14 |
| 19 | Musk, Flannery | SO | Bellarmine | 5:25.45 |
| 20 | Wilhelm, Katherine | JR | Indianapolis | 5:27.92 |
| 21 | Kerschieter, Bailey | FR | Wis.-Parkside | 5:30.11 |
| 22 | Baughman, Haley | FR | Indianapolis | 5:32.64 |
| 23 | Varela, Brenda | SR | Drury | 5:33.69 |
| 24 | Mauch, Emily | FR | Maryville (Mo.) | 5:35.93 |
| 25 | Wingert, Carly | SO | Drury | 5:38.65 |
| 26 | Kearney, Sarah | JR | Lewis | 5:39.20 |
| 27 | Dukewits, Lindsay | SR | Drury | 5:40.25 |
| 28 | Woodbury, Leah | SO | Southern Indiana | 5:42.28 |
| 29 | Adkins, Jamie | FR | Southern Indiana | 5:51.63 |
| 30 | Kriz, Maddie | FR | Southern Indiana | 6:00.10 |