| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | Musk, Angela | SR | Bellarmine | 2:15.64 |
| 2 | Miller, Haley | SR | Indianapolis | 2:17.11 |
| 3 | Sebby, Krysten | JR | Lewis | 2:18.50 |
| 4 | Zittleman, Amanda | SO | Wis.-Parkside | 2:19.20 |
| 5 | Roark, Madison | JR | Bellarmine | 2:19.32 |
| 6 | Lee, Jenna | SR | Bellarmine | 2:20.43 |
| 7 | Wagner, Maggie | SR | Bellarmine | 2:20.91 |
| 8 | Dickerson, Taylor | FR | Maryville (Mo.) | 2:22.25 |
| 9 | Mornout, Jordan | JR | Southern Indiana | 2:22.33 |
| 10 | Thorton, Emily | FR | Indianapolis | 2:22.39 |
| 11 | Wojan, Katelyn | JR | Drury | 2:22.66 |
| 12 | Cotherman, Taylor | FR | Indianapolis | 2:24.02 |
| 13 | Polhemus, Amy | SO | Lewis | 2:24.14 |
| 14 | Green, Keagin | SO | Indianapolis | 2:24.25 |
| 15 | Updyke, Natalie | SR | Missouri S&T | 2:24.61 |
| 16 | Wilmes, Maggie | JR | Drury | 2:24.88 |
| 17 | DeBoy, Katie | JR | St. Joseph's (Ind.) | 2:25.47 |
| 18 | Clawson, Amy | JR | Lewis | 2:27.73 |
| 19 | Boyle, Allison | FR | St. Joseph's (Ind.) | 2:28.76 |
| 20 | Shockley, Jessica | FR | William Jewell | 2:29.14 |
| 21 | Cunningham, Christine | SO | McKendree | 2:29.66 |
| 22 | Reagan, McKenzie | JR | William Jewell | 2:29.95 |
| 23 | Landolt, Ciera | SR | McKendree | 2:31.33 |
| 24 | Pettz, Shelby | SR | William Jewell | 2:31.98 |
| 25 | Jarman, Deidra | JR | Maryville (Mo.) | 2:33.27 |
| 26 | Kearney, Sarah | JR | Lewis | 2:34.57 |
| 27 | Nichols, Shorrae | JR | McKendree | 2:43.43 |
| 28 | Senaldi, Alex | SR | McKendree | 2:54.08 |