| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | Kindred, Jermel | JR | Indianapolis | 49.23 |
| 2 | Gardner, III, Robert | JR | Indianapolis | 49.63 |
| 3 | Ederer, Clint | SR | William Jewell | 50.01 |
| 4 | Flournoy, Jerel | SR | Indianapolis | 50.11 |
| 5 | Karwowski, Michael | SR | Wis.-Parkside | 50.41 |
| 6 | Oldham, Cody | FR | Bellarmine | 50.50 |
| 7 | Schwartz, Kyle | SO | Indianapolis | 50.75 |
| 8 | Flannagan, Collin | FR | Bellarmine | 51.21 |