| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | Kindred, Jermel | JR | Indianapolis | 48.45 |
| 2 | Gardner, III, Robert | JR | Indianapolis | 48.53 |
| 3 | Flournoy, Jerel | SR | Indianapolis | 48.72 |
| 4 | Ederer, Clint | SR | William Jewell | 49.12 |
| 5 | Karwowski, Michael | SR | Wis.-Parkside | 49.52 |
| 6 | Klosterman, Sean | SR | Bellarmine | 49.70 |
| 7 | Armstrong, Justin | SO | Wis.-Parkside | 50.06 |
| 8 | Flannagan, Collin | FR | Bellarmine | 50.63 |
| 9 | Oldham, Cody | FR | Bellarmine | 50.83 |
| 10 | Schwartz, Kyle | SO | Indianapolis | 50.83 |
| 11 | Thompson, Nick | SR | McKendree | 50.94 |
| 12 | Stark, Jordan | JR | McKendree | 50.95 |
| 13 | Smith, Travis | SR | Missouri S&T | 51.21 |
| 14 | Bailey, Brendan | FR | Wis.-Parkside | 51.83 |
| 15 | Galloway, Cole | JR | St. Joseph's (Ind.) | 51.89 |
| 16 | Fieber, Zack | FR | McKendree | 52.75 |
| 17 | Lockhart, Eddie | JR | Wis.-Parkside | 54.44 |
| 18 | Patterson, Justin | SR | Lewis | 55.68 |
| 19 | Harris, Nehiamah | SO | Lewis | NT |
| 20 | Edwards, David | JR | Lewis | NT |
| 21 | Randich, Moses | FR | McKendree | NT |