| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | Martin, Deltarae | SR | Indianapolis | 59.14 |
| 2 | Jones, Meghan | JR | Bellarmine | 59.29 |
| 3 | Martin, LaTisha | SO | Indianapolis | 59.44 |
| 4 | Swenson, Maggie | JR | William Jewell | 59.92 |
| 5 | Rawson, Rachel | SO | Bellarmine | 1:00.65 |
| 6 | Lawal, Rachel | FR | Missouri S&T | 1:00.97 |
| 7 | Jackson, Jasmin | FR | Indianapolis | 1:01.31 |
| 8 | Meier, Alex | FR | St. Joseph's (Ind.) | 1:01.36 |
| 9 | Johnson, Curshonne | FR | Wis.-Parkside | 1:01.39 |
| 10 | Owens, Kenya | SR | Indianapolis | 1:01.46 |