| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | Jones, Meghan | JR | Bellarmine | 56.73 |
| 2 | Martin, LaTisha | SO | Indianapolis | 57.23 |
| 3 | Martin, Deltarae | SR | Indianapolis | 57.65 |
| 4 | Swenson, Maggie | JR | William Jewell | 57.88 |
| 5 | Owens, Kenya | SR | Indianapolis | 59.11 |
| 6 | Johnson, Curshonne | FR | Wis.-Parkside | 59.67 |
| 7 | Goodwin, Tori | FR | Bellarmine | 59.93 |
| 8 | Weis, Aryn | FR | Maryville (Mo.) | 1:00.12 |
| 9 | Hopkins, Breanna | FR | Wis.-Parkside | 1:00.23 |
| 10 | Rawson, Rachel | SO | Bellarmine | 1:00.52 |
| 11 | Bowe, Nora | SR | Bellarmine | 1:00.64 |
| 12 | Jackson, Jasmin | FR | Indianapolis | 1:00.65 |
| 13 | Meier, Alex | FR | St. Joseph's (Ind.) | 1:00.89 |
| 14 | Lawal, Rachel | FR | Missouri S&T | 1:00.92 |
| 15 | Carbajal, Karina | SR | Lewis | 1:01.04 |
| 16 | Szilard, Molly | SR | Lewis | 1:01.67 |
| 17 | Reppell, Gabrielle | SO | Drury | 1:02.25 |
| 18 | Hemmerling, Kristina | JR | St. Joseph's (Ind.) | 1:02.68 |
| 19 | Dellahan, Dayton | FR | St. Joseph's (Ind.) | 1:03.67 |
| 20 | Voltaire, Kim | FR | Lewis | 1:04.47 |
| 21 | Gerber, Jessica | FR | McKendree | 1:04.97 |
| 22 | Nichol, Meghan | SO | Lewis | 1:05.96 |