| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | Hawkins, Adriel | JR | Missouri S&T | 21.64 |
| 2 | Kindred, Jermel | JR | Indianapolis | 21.79 |
| 3 | Flournoy, Jerel | SR | Indianapolis | 21.81 |
| 4 | Gardner, III, Robert | JR | Indianapolis | 21.82 |
| 5 | Qualls, Quinntyn | FR | Indianapolis | 21.84 |
| 6 | Karwowski, Michael | SR | Wis.-Parkside | 22.05 |
| 7 | McKinney, Nathan | JR | Bellarmine | 22.24 |
| 8 | Williams, Damar | JR | Bellarmine | 22.34 |
| 9 | Ederer, Clint | SR | William Jewell | 22.41 |
| 10 | Dorenkamper, Kevin | SO | Lewis | 22.52 |
| 11 | James, Cameron | FR | McKendree | 22.52 |
| 12 | Murr, Brian | FR | St. Joseph's (Ind.) | 22.53 |
| 13 | Stark, Jordan | JR | McKendree | 22.58 |
| 14 | Martin, Sean | SR | Missouri S&T | 22.61 |
| 15 | Blandon, Lamarr | JR | Lewis | 22.63 |
| 16 | Lengsfeld, Peter | SO | Lewis | 23.00 |
| 17 | Taylor, Michael | JR | St. Joseph's (Ind.) | 23.32 |
| 18 | Duncan, Dallas | FR | Drury | 23.34 |
| 19 | Smith, Travis | SR | Missouri S&T | 23.43 |
| 20 | White, Corey | FR | St. Joseph's (Ind.) | 23.56 |
| 21 | Vetter, Brady | FR | Missouri S&T | 23.57 |
| 22 | Greco, Michael | JR | Lewis | 23.93 |
| 23 | Eubanks, David | FR | St. Joseph's (Ind.) | 24.07 |
| 24 | Bowers, Dillon | FR | Maryville (Mo.) | 24.18 |
| 25 | Cooper, Zach | FR | Southern Indiana | 24.27 |
| 26 | Thompson, Michael | SO | Maryville (Mo.) | 24.33 |
| 27 | Roediger, Nick | JR | Maryville (Mo.) | 26.65 |
| 28 | Owens, Steve | SO | Maryville (Mo.) | NT |