| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | Jones, Meghan | JR | Bellarmine | 25.16 |
| 2 | O'Bannon, Kaysee | FR | Bellarmine | 25.34 |
| 3 | Martin, Deltarae | SR | Indianapolis | 25.79 |
| 4 | Ferguson, Kamille | SR | Lewis | 25.81 |
| 5 | Owens, Kenya | SR | Indianapolis | 25.87 |
| 6 | Martin, LaTisha | SO | Indianapolis | 25.97 |
| 7 | King, Kayla | SO | Lewis | 26.05 |
| 8 | Johnson, Curshonne | FR | Wis.-Parkside | 26.26 |