| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | Ferguson, Kamille | SR | Lewis | 24.52 |
| 2 | Jones, Meghan | JR | Bellarmine | 24.99 |
| 3 | O'Bannon, Kaysee | FR | Bellarmine | 25.02 |
| 4 | Martin, LaTisha | SO | Indianapolis | 25.67 |
| 5 | Martin, Deltarae | SR | Indianapolis | 25.85 |
| 6 | Johnson, Curshonne | FR | Wis.-Parkside | 25.95 |
| 7 | Owens, Kenya | SR | Indianapolis | 26.01 |
| 8 | King, Kayla | SO | Lewis | 26.03 |
| 9 | Wrocklage, Olivia | SO | Bellarmine | 26.13 |
| 10 | Bradshaw, Mary | SR | Missouri S&T | 26.15 |
| 11 | Wentzloff, Amber | JR | Lewis | 26.26 |
| 12 | Meier, Katlyn | FR | Missouri S&T | 26.32 |
| 13 | Perkins, Danielle | SO | St. Joseph's (Ind.) | 26.33 |
| 14 | Weis, Aryn | FR | Maryville (Mo.) | 26.49 |
| 15 | Rascoe, Lauren | JR | Indianapolis | 26.54 |
| 16 | Ledbetter, Tennia | JR | St. Joseph's (Ind.) | 26.92 |
| 17 | Guillaume, Kothney-Issa | JR | William Jewell | 26.97 |
| 18 | Blythe, Erica | SR | St. Joseph's (Ind.) | 27.00 |
| 19 | Stacy, Taylor | SO | St. Joseph's (Ind.) | 27.93 |
| 20 | Klott, Jodi | SO | Maryville (Mo.) | 28.20 |
| 21 | McGee, Tiarra | FR | Lewis | 28.88 |
| 22 | Clark, Tiffany | JR | Kentucky Wesleyan | 33.39 |
| 23 | Brennan, Dana | SR | Maryville (Mo.) | NT |