| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | O'Bannon, Kaysee | FR | Bellarmine | 07.66 |
| 2 | King, Kayla | SO | Lewis | 07.71 |
| 3 | Ferguson, Kamille | SR | Lewis | 07.77 |
| 4 | Wrocklage, Olivia | SO | Bellarmine | 07.89 |
| 5 | Blythe, Erica | SR | St. Joseph's (Ind.) | 07.99 |
| 6 | Meier, Katlyn | FR | Missouri S&T | 08.03 |
| 7 | Edwards, Camille | JR | Indianapolis | 08.03 |
| 8 | Perkins, Danielle | SO | St. Joseph's (Ind.) | 08.08 |
| 9 | McGregor, Shanese | SR | Indianapolis | 08.08 |
| 10 | Kuntz, Elaine | SR | Bellarmine | 08.09 |
| 11 | Wentzloff, Amber | JR | Lewis | 08.11 |
| 12 | Rascoe, Lauren | JR | Indianapolis | 08.18 |
| 13 | Johnson, Cidnei | FR | Bellarmine | 08.25 |
| 14 | Guillaume, Kothney-Issa | JR | William Jewell | 08.26 |
| 15 | Ford, Lynn | FR | McKendree | 08.26 |
| 16 | Klott, Jodi | SO | Maryville (Mo.) | 08.46 |
| 17 | Stacy, Taylor | SO | St. Joseph's (Ind.) | 08.54 |
| 18 | Johnson, Krystin | JR | Indianapolis | 08.70 |
| 19 | Martinez, Daisy | FR | Lewis | 08.98 |