| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | Edwards, Camille | JR | Indianapolis | 08.86 |
| 2 | Marchildon, Megan | JR | Lewis | 08.97 |
| 3 | Stewart, Samantha | JR | Lewis | 09.01 |
| 4 | Nirva, Samantha | SO | St. Joseph's (Ind.) | 09.05 |
| 5 | Jackson, Jasmin | FR | Indianapolis | 09.15 |
| 6 | Bowe, Nora | SR | Bellarmine | 09.18 |
| 7 | Goodwin, Tori | FR | Bellarmine | 09.27 |
| 8 | Atkins, Alexis | SR | Lewis | 09.39 |
| 9 | Ford, Lynn | FR | McKendree | 09.39 |
| 10 | Ulsas, Haley | SO | Maryville (Mo.) | 09.41 |
| 11 | Perko, Margaret | FR | William Jewell | 09.50 |
| 12 | Meier, Alex | FR | St. Joseph's (Ind.) | 09.59 |
| 13 | Morgan, Amani | FR | Indianapolis | 09.65 |
| 14 | Roark, Madison | JR | Bellarmine | 09.70 |
| 15 | Raypole, Corinna | SO | Indianapolis | 09.78 |
| 16 | Hemmerling, Kristina | JR | St. Joseph's (Ind.) | 10.10 |