| Athlete | Yr | Team | Seed | |
|---|---|---|---|---|
| 1 | Sandlin, Robert | JR | Bellarmine | 4:05.74 |
| 2 | Schickel, Tyler | FR | Southern Indiana | 4:12.69 |
| 3 | Jemilo, Matt | SO | Lewis | 4:14.10 |
| 4 | McMillan, Benjamin | JR | Indianapolis | 4:14.55 |
| 5 | Callison, Michael | SR | Southern Indiana | 4:15.47 |
| 6 | DeLong, Blake | JR | McKendree | 4:15.59 |
| 7 | Dotterweich, Nate | SR | Bellarmine | 4:18.12 |
| 8 | Escareno, Jesus | SR | Wis.-Parkside | 4:19.88 |
| 9 | Barrett, Logan | SO | St. Joseph's (Ind.) | 4:19.89 |
| 10 | Stocker, Kyle | SO | Missouri S&T | 4:20.59 |
| 11 | Whitehead, Michael | SR | Southern Indiana | 4:20.92 |
| 12 | O'Brien, Sean | SO | Indianapolis | 4:20.98 |
| 13 | Zuniga, Joe | SO | Maryville (Mo.) | 4:22.55 |
| 14 | Mikkelson, Jimmy | SO | Wis.-Parkside | 4:23.23 |
| 15 | Boyle, Zach | SO | Maryville (Mo.) | 4:24.60 |
| 16 | Armstrong, Matt | JR | Drury | 4:26.54 |
| 17 | Parks, Cody | FR | Bellarmine | 4:27.19 |
| 18 | Abernathy, Kevin | JR | Drury | 4:28.17 |
| 19 | Marchman, Chris | SO | Missouri S&T | 4:28.32 |
| 20 | Wagner, Sean | FR | Wis.-Parkside | 4:28.70 |
| 21 | Voss, Colin | SR | McKendree | 4:28.72 |
| 22 | Stone, Cody | FR | Southern Indiana | 4:29.18 |
| 23 | Villarreal, Josue | SR | William Jewell | 4:30.68 |
| 24 | Keller, Joel | FR | Indianapolis | 4:31.47 |
| 25 | Craven, Austin | FR | Drury | 4:31.81 |
| 26 | Heady, Uriah | FR | William Jewell | 4:33.56 |
| 27 | Weissensel, James | FR | Lewis | 4:35.05 |
| 28 | Walker, Allen | SO | Lewis | 4:35.70 |
| 29 | Georgescu, Vincent | JR | Indianapolis | 4:36.03 |
| 30 | Witt, Brian | SR | St. Joseph's (Ind.) | 4:37.52 |
| 31 | Daniel, Mitch | FR | Drury | 4:43.73 |
| 32 | Allen, Anthony | FR | Maryville (Mo.) | NT |