| Lane | Athlete | Team | Seed | 
|---|---|---|---|
| 3 | Widder, Hailey | Olivet Nazarene | 1:10.00 | 
| 4 | Ertel, Kerigan | Indianapolis | 1:04.47 | 
| 5 | Sutton, Harley | Calumet-St. Joseph | 1:07.24 | 
| 6 | Spaulding, Hope | Marian (Ind.) | 1:11.03 | 
| Lane | Athlete | Team | Seed | 
|---|---|---|---|
| 3 | Parsell, Courtney | Southern Indiana | 1:12.02 | 
| 4 | McGinnis, Abigail | Olivet Nazarene | 1:11.12 | 
| 5 | Kasper, Kylee | Marian (Ind.) | 1:11.22 | 
| 6 | Kurcz, Maddie | Marian (Ind.) | 1:12.50 |