| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MANN, Cade | SO | Emporia St. |
49.35 | MIAA Outdoor Championships |
| 2 | DONELSON, Sean | FR | 48.13 | raw: 48.02 2025 RMAC Outdoor Track & Field Championship | |
| 3 | WHISLER, Ashton | SO | 47.11 | raw: 47.00 2025 RMAC Outdoor Track & Field Championship | |
| 4 | GRIFFIN III, Keith | FR | NW Missouri |
46.99 | Un. of Central Missouri Mule Relays |
| 5 | GREENE, Kenny | SO | MSU Denver |
47.00 | raw: 46.89 2025 RMAC Outdoor Track & Field Championship |
| 6 | PRATO, Zavio | SO | Mount Marty |
48.02 | 2025 GPAC Outdoor Track and Field Championships |
| 7 | NELSON, Peyton | FR | 48.41 | raw: 48.30 2025 RMAC Outdoor Track & Field Championship | |
| 8 | JANTZEN, Danny | Unattached | 49.50 | 49.5 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | GOECKEL, Matt | Tallgrass Track Club | 51.60 | 51.60 | |
| 3 | ANDERSON, Braden | JR | Black Hills St. |
50.50 | raw: 53.24 TFRRS 01/13/24 - Braden%27s 400m indoor PR is from him racing it as part of a workout indoors last year. He goes through the first lap of his 800 in 53-53.7 consistently%2C so if I can enter a speculative%2C I%27d enter him at 49.00 |
| 4 | DIAMOND, Carter | FR | Nebraska-Kearney |
49.76 | 2025 Loper Invitational |
| 5 | O'LEARY, Aiden | FR | Black Hills St. |
50.30 | raw: 50.19 CU Invitational |
| 6 | WALDMANN, Brady | FR | Nebraska-Kearney |
51.44 | 2025 Loper Invitational |
| 7 | BESSMER, TJ | JR | MidAmerica Nazarene |
51.65 | Zack Kindler Wildcat Open |