| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Emporia St. |
A | Bennett | Anderson | Domeh | Mollerus | DNF | Concordia Twilight |
| 4 | Chadron State |
A | Iyengunmwena | Cormier | Clapper | Sup | 39.88 | TFRRS 05/11/24 - 40.90 |
| 5 | Fort Hays St. |
A | Albright | Wahlmeier | Albright | Churchman | 40.23 | 2025 Central Nebraska Challenge |