| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Iowa Western CC |
C | Nelson | Hartnett | Baez-Mahr | Tannehill-Downey | DNS | |
| 3 | Iowa Western CC |
A | Baez-Mahr | Mabar | Nelson | Mendez | 3:35.67 | |
| 4 | Nebraska-Kearney |
A | Diamond | Deer | Waldmann | Emsick | 3:16.00 | |
| 5 | Nebraska-Kearney |
B | Diamond | Waldmann | Daniels | Drahota | DNS | |
| 6 | Iowa Western CC |
B | Hartley | Turner | Hartnett | Flohr | 3:35.67 |