| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FOLDS, Alyssa | FR | Wayne State (Neb.) |
25.98 | South Dakota Challenge |
| 2 | OSTRAND, Ashley | SR | Omaha |
25.73 | Summit League Outdoor Championships |
| 3 | BARNARD, Regan | FR | Omaha |
25.35 | 2025 Central Nebraska Challenge |
| 4 | KOUMEDZINA, Judith | SO | Iowa Western CC |
24.47 | Ichabod Invitational |
| 5 | FRANKLIN, Aaliyah | SO | Nebraska-Kearney |
25.34 | 2025 Central Nebraska Challenge |
| 6 | MADER, Reba | JR | Fort Hays St. |
25.58 | Emporia State MIdwest Classic |
| 7 | FOSTER, Amelia | SO | Fort Hays St. |
25.87 | raw:25.80 Jo Meaker Classic and Multi |
| 8 | ROSE, Abby | JR | Nebraska-Kearney |
26.03 | 2024 Central Nebraska Challenge |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | VANCE, Molly | SO | Colorado College |
28.07 | raw:28.00 Chuck Haering Classic |
| 2 | MUSTARD, Grace | JR | Omaha |
27.60 | Jim Emmerich Alumni Meet |
| 3 | BRUNOMUNN, Zaniya | FR | Colorado College |
27.31 | 2025 War Hawk Classic |
| 4 | BARNES, Aubrey | FR | Nebraska-Kearney |
27.05 | 2025 Central Nebraska Challenge |
| 5 | JONES, Emma | JR | Colorado College |
27.26 | Wichita State KT Woodman Classic |
| 6 | SCHMIDT, Koral | SO | Nebraska-Kearney |
27.49 | Concordia Twilight |
| 7 | KIRR, Clara | FR | Colorado College |
27.98 | 2025 War Hawk Classic |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | DEMKE, Madison | FR | Nebraska Wesleyan |
NT | |
| 4 | MIRANDA, Alina | SO | Colorado College |
28.63 | Wichita State KT Woodman Classic |
| 5 | FLAMING, Kendyl | FR | Nebraska-Kearney |
28.63 | 2025 Concordia Classic |