| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIAMOND, Carter | FR | Nebraska-Kearney |
51.08 | 2025 Bearcat Invite |
| 2 | MADSEN, Austin | JR | Wayne State (Neb.) |
50.55 | Mount Marty Twilight |
| 3 | ALBRIGHT, Avrey | FR | Fort Hays St. |
48.35 | 2025 MIAA Indoor Championships |
| 4 | KULHANEK, Will | FR | Nebraska-Kearney |
47.65 | 2025 Central Nebraska Challenge |
| 5 | EDWARDS, Jordan | FR | Iowa Western CC |
47.81 | raw:47.70 NJCAA Championships |
| 6 | DEER, Mitchell | JR | Nebraska-Kearney |
48.43 | 2024 Concordia Outdoor Invite |
| 7 | COOK, Jalen | SR | Briar Cliff |
50.70 | Sioux City Relays UCO 2025 |
| 8 | BURNS, Brody | FR | Fort Hays St. |
51.20 | Emporia State MIdwest Classic |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WASSOM, Asher | FR | Fort Hays St. |
52.32 | 2025 Washburn Rust Buster |
| 2 | SIEGEL, Mathew | JR | Nebraska Wesleyan |
51.91 | SDSU Indoor Classic |
| 3 | WALDMANN, Brady | FR | Nebraska-Kearney |
51.73 | Wichita State KT Woodman Classic |
| 4 | RUCKER, Jayson | JR | Briar Cliff |
51.24 | 17th Annual Wayne State -Wildcat Classic 2025 |
| 5 | DUBA, Cole | JR | Nebraska Wesleyan |
51.42 | Prairie Wolf Indoor Invitational 2025 |
| 6 | RUDIE, Mitchell | JR | Wayne State (Neb.) |
51.78 | WSC Wildcat Open |
| 7 | DARLING, Cole | SR | Fort Hays St. |
51.93 | Ichabod Invitational |
| 8 | ARNOLD, Alex | FR | Fort Hays St. |
52.65 | Emporia State MIdwest Classic |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | PENG, Andrew | SO | Colorado College |
54.00 | |
| 3 | TODD-TARADAY, Asher | JR | Colorado College |
52.96 | SCAC Outdoor Track & Field Championships |
| 4 | NEVAREZ, Dylan | FR | Colorado College |
52.87 | raw:52.76 Chuck Haering Classic |
| 5 | KURTZ, Dylan | SR | Nebraska Wesleyan |
52.90 | SDSU Indoor Classic |
| 6 | JOHNSON, Malcolm | FR | Unattached | 53.35 | |
| 7 | DISNEY, Aidan | JR | Colorado College |
55.70 | raw:55.59 CU Invitational |