| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 4 | SCHMIDT, Koral | FR | Nebraska-Kearney |
1:01.87 | 2024 Loper Twilight |
| 5 | DILLAN, Kamrie | SR | York (Neb.) |
58.44 | 2024 KCAC Outdoor Championship |
| 6 | IRVIN, Ty | SR | York (Neb.) |
1:01.77 | 2024 KCAC Outdoor Championship |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 5 | FLAMING, Kendyl | FR | Nebraska-Kearney |
NT | |
| 6 | JOHNSON, Felicity | SO | York (Neb.) |
NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 5 | REESE, Ava | FR | Hastings |
NT | |
| 6 | TUCKER, Lauren | FR | York (Neb.) |
NT |